嗜热红藻藻胆体光热稳定性研究与应用
李凯伦，李文军，马建飞*，秦 松*
（1.中国科学院烟台海岸带研究所，山东 烟台 264003；2.中国科学院大学，北京 100049；3.南开大学药物化学生物学全国重点实验室，生命科学学院，天津 300350）
摘 要 藻胆体作为蓝藻和红藻最主要的捕光蛋白复合体，主要功能是捕获光能和传递到类囊体膜内的光系统。本文研究了嗜热红藻藻胆体在高光强下的光稳定性和高温下的热稳定性，以嗜温红藻紫球藻的藻胆体作对照，不同时间梯度处理之后，测定二者蛋白功能完整性变化。结果表明，嗜热红藻在高光强和高温下的光热稳定性均比嗜温红藻要强，其光热稳定的性质，使得嗜热红藻藻胆体适合于应用于太阳能下的产光热的环境中，应用在光电转换的人工光合作用器件中。
关键词 嗜热红藻；藻胆体；光稳定性；热稳定性
中图分类号：Q51；Q71；Q336 文献标识码：ADoi:10.3969/j.issn.1000-6281.2025.01.007
Study and application of photothermal stability of the thermophilic red alga phycobilisome
LI Kailun#，LI Wenjun，MA Jianfei*，QIN Song*
（1.Yantai Institute of Coastal Zone，Chinese Academy of Sciences，Yantai Shandong 264003；2.University of Chinese Academy of Sciences，Beijing 100000；3.State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences，Nankai University，Tianjin 300350，China）
Abstract Phycobilisomes represent the primary light-harvesting protein complexes in cyanobacteria and red algae. They capture light energy and transfer it to the photosystems in the thylakoid membrane. This study investigates the photostability of phycobilisomes from thermophilic red algae under high light intensity and their thermal stability at elevated temperatures, employing phycobilisomes from the mesophilic red algae Porphyridium purpureum as a control. Following the completion of the designated treatment periods, an evaluation of the functional integrity of the proteins in both samples was conducted. The findings indicate that thermophilic red algae exhibit markedly elevated photothermal stability under conditions of elevated light and temperature when compared to mesophilic red algae. This enhanced photothermal stability suggests that phycobilisomes from thermophilic red algae are optimally suited for utilisation in solar-driven, heat-generating environments, offering a promising avenue for incorporation into photothermal conversion devices for artificial photosynthesis.
Keywords thermophilic red algae；phycobilisome；photostability；thermal stability
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