无相互作用自由空间电子光学与量子电子显微镜

无相互作用自由空间电子光学与量子电子显微镜

 赵利荣，崔益民，彭腾飞，李文萍^*

（北京航空航天大学，北京 100191）

摘  要   本文围绕着生物等敏感样品的高分辨透射电子显微成像展开，从电子显微成像等方面论述了透射电子显微镜在敏感样品领域的发展，重点介绍了基于无相互作用测量（IFM）的量子电子相干成像机理，分析了自由空间电子光学中电子IFM对耦合相干电子源和耦合电子传输的要求，量子机制和电子显微镜相结合建立的量子电子显微镜能够从本质上解决高能电子辐照对生物等敏感样品的损伤，将成为敏感样品高分辨无损检测发展史上的里程碑。

关键词     透射电子显微镜；无相互作用测量；自由空间电子光学；量子电子显微镜

中图分类号：TN16；O46  文献标识码: A              doi：10.3969/j.issn.1000-6281.2024.03.013

Comparison of sample preparation methods and observation on salt glands of Tamarix using scanning electron microscopy

YIN Dandan¹, WANG Liuqiang², CAO Yuan¹, YUE Zhiqiang^1*

(1.State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091；2. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China)

Abstract  In this study, the leaves of tissue and soil culture seedlings of Tamarix hispida were pretreated by CO₂ critical point dryingmethod and Cryo-scanning electron microscope (SEM) sample preparation method. The morphological characteristics of salt glands were observed and compared by SEM. The results showed that the effect of Cyro-SEM method was better than that of CO₂ critical point drying method for young tissue culture seedlings. For fleshy soil culture seedlings, the CO₂ critical point drying method was better than the Cyro-SEM method. Subsequently, this study conducted the CO₂ critical point drying method and SEM observation on the leaves of soil culture seedlings of 10 Tamarix species, and counted the number and area of salt glands. The results showed that T.elongata had the highest number and area of salt glands. The advantage and disadvantage of the two sample preparation methods was presented based on the salt gland morphology of Tamarix, and the salt glands of 10 Tamarix leaves were further studied. This work provides a reference for the basic and applied research on salt resistance of Tamarix.

keywords  scanning electron microscope; salt gland; Tamarix; sample preparation method

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