一种用于冷冻电镜样品装载的工作台*
饶桂波一种用于冷冻电镜样品装载的工作台*
饶桂波
(中国科学院武汉病毒研究所,湖北 武汉 410000)
摘 要 本文介绍一种自行设计用于冷冻电镜样品装载的工作台。在保持操作区域相对低温及低气流扰动下,其可以将湿度降至10%以内。在该操作台里完成装载的冷冻样品,几乎没有冰晶污染,可用于冷冻电镜高分辨数据采集。
关键词 冷冻电镜;样品装载;冰晶污染
中图分类号:Q816,Q71;TP23;Q33 文献标识码:A doi:10.3969/j.issn.1000-6281.2024.02.012
A bench for loading Cryo-electron microscope samples
RAO Guibo
(Wuhan institute of virology CAS, Wuhan Hubei 410000, China)
Abstract In this paper, a bench for loading Cryo-electron microscope samples was presented. The bench reduced the humidity in the operating area to less than 10 % while maintained a relatively low temperature and a low airflow disturbance. The frozen samples loaded in the bench had almost no ice crystal contamination, and could be used for high-resolution data acquisition of Cryo-electron microscope.
Keywords Cryo-electron microscope; sample loading; ice crystal contamination
“全文下载请到同方知网,万方数据库或重庆维普等数据库中下载!”