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灵武长枣果实阿拉伯半乳糖蛋白免疫荧光定位

王 静， 章英才*， 陶珊珊
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2022年 第41卷 第3期: 1000-6281（2022）03-293-05     下载地址：

灵武长枣果实阿拉伯半乳糖蛋白免疫荧光定位

王  静， 章英才*， 陶珊珊

（宁夏大学生命科学学院，宁夏银川750021）

摘  要   目的：为探讨灵武长枣果实AGPs糖蛋白在果实发育过程中的分布特征和动态变化规律。方法：以4个时期灵武长枣果实为实验材料，通过免疫组织化学方法，研究了不同发育时期果实阿拉伯半乳糖蛋白AGPS的分布，为进一步研究灵武长枣果实AGPs在品质调控方面的功能奠定基础。结果：（1）JIM13和JIM8抗体所识别的抗原在4个时期果实的外果皮及相邻的内部数层排列紧密的中果皮小细胞细胞壁和细胞内部均有分布。（2）除了空腔之外，内部的中果皮大型卵圆形薄壁细胞的细胞壁和细胞内在膨大前期均有JIM13和JIM8抗体所识别的抗原分布，而快速膨大期、着色期和完熟期果实JIM13和JIM8抗体所识别的抗原均主要分布于细胞壁上，大部分细胞内部无分布；随着果实发育成熟，空腔数量增多，中果皮薄壁细胞间隙拉大排列更加松散，出现细胞破裂，JIM13和JIM8抗体所识别的抗原分布逐渐减少。（3）4个时期果实维管束中维管束鞘、木质部、韧皮部、形成层所有细胞的细胞壁和细胞内部都分布有JIM13和JIM8抗体所识别的抗原，维管束数量和大小随果实发育及体积的进一步增大逐渐减少，JIM13和JIM8抗体所识别的抗原分布逐渐减少。结论：JIM13和JIM8为灵武长枣果实免疫组织化学的良好抗体，各时期果实的不同组织JIM13和JIM8抗体所识别的抗原荧光强弱存在一定的差异；AGPs参与了灵武长枣果实维管束发育过程的形态建成、细胞的分裂和体积的增大，为果实发育提供营养支持及保护。

关键词   灵武长枣；果实；阿拉伯半乳糖蛋白；免疫荧光定位

中图分类号：Q942.6；Q944.62；Q513；S665.1  文献标识码：Adoi:10.3969/j.issn.1000-6281.2022.03.011

 

Immunofluorescence localization of arabinogalactan proteins inZiziphus jujuba Mill cv. Lingwuchangzao fruit

WANG Jing，ZHANG Ying-cai*，TAO Shan-shan

（School of Life Science，Ningxia University，Yinchuan Ningxia 750021，China）

Abstract   Objective:To uncover the distribution characteristics and dynamic changes of AGPs in Ziziphus jujuba Mill cv. Lingwuchangzao fruit during fruit development.Methods:The distribution of arabinogalactan proteins AGPS was studied by immunohistochemistry in four periods ofZiziphus jujuba Mill cv. Lingwuchangzao fruit, laying the foundation for further research on the functions of AGPs in quality regulation of Ziziphus jujuba Mill cv. Lingwuchangzao fruit. Results: (1) The antigens recognized by JIM13 and JIM8 antibodies were distributed in the cell walls and cell interiors of the epicarp and the alongside internal layers of the closely-arranged mesocarp small cells in four periods fruit. (2) Except for the cavities, the antigens recognized by JIM13 and JIM8 antibodies were distributed in the cell walls and the inner part of the large ovoid parenchyma cells in the inner mesocarp during the early bulking period, while the antigens recognized by JIM13 and JIM8 antibodies were mainly distributed in the cell walls during the rapid enlargement period, coloring period and maturation periods, with no antigens distribution in the inner part of most cells. As the fruit developed and matured the number of cavities increased, the mesocarp parenchyma cells became more loosely arranged with elongated intercellular spaces. Cell rupture occurred, and the distribution of antigens recognized by JIM13 and JIM8 antibodies gradually decreased. (3) The antigens recognized by JIM13 and JIM8 antibodies were distributed in the cell walls and cell interiors of all cells in the vascular bundle sheaths, xylem, phloem, and cambium of vascular bundles in four periods fruit, and the number and size of vascular bundles gradually decreased with fruit development. Further increase in size, and the distribution of antigens recognized by JIM13 and JIM8 antibodies gradually decreased. Conclusion:JIM13 and JIM8 are good antibodies for immunohistochemistry in Ziziphus jujuba Mill cv. Lingwuchangzao fruit, and there are some differences in the fluorescence intensity of antigens recognized by JIM13 and JIM8 antibodies in different tissues of fruit at different periods, AGPs participate in morphogenesis, cell division and volume increase during vascular bundles development of Ziziphus jujuba Mill cv. Lingwuchangzao fruit, which provides nutritional support and protection for fruit development.

Keywords  Ziziphus jujuba Mill cv. Lingwuchangzao；fruit；arabinogalactan proteins；immunofluorescence localization

 

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