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子宫内膜异位症细胞微尺度刚度的初步实验研究

李 霞，冯建涛，任克礼，孙全梅，赵小玲，韩 东，孔为民*
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2021年 第40卷 第1期: 1000-6281（2021）01-037-04     下载地址：

子宫内膜异位症细胞微尺度刚度的初步实验研究

李  霞，冯建涛，任克礼，孙全梅，赵小玲，韩  东，孔为民*

（1.北京航天总医院，北京100076；2.中国中医科学院青蒿素研究中心，北京100700；3.国家纳米科学中心，北京100190；4.首都医科大学附属北京妇产医院妇瘤科，北京100006）

摘  要 目的：基于原子力显微技术，建立子宫内膜异位症细胞力学性质检测方法，比较子宫内膜异位症在位与异位子宫内膜细胞的微尺度刚度，以进一步研究子宫内膜异位症发病机制。方法：采集子宫内膜异位症患者子宫内膜及其异位到卵巢的巧克力囊肿囊皮，并分别进行原代培养，利用原子力显微镜采集细胞力曲线，获取杨氏模量信息，进行统计分析。结果：在位腺上皮细胞的杨氏模量高于异位腺上皮细胞（2.96±1.48 kPa，1.47±1.15 kPa，P<0.05）；在位间质细胞与异位间质细胞杨氏模量比较无明显变化（3.93±1.50kPa，3.14±1.65kPa，P>0.05）。结论：子宫内膜在位腺上皮细胞与异位腺上皮细胞力学特性不同，异位腺上皮细胞刚度降低，因此推论细胞刚度小更有利于细胞的迁移，本研究结果支持细胞迁移学说。

关键词 子宫内膜异位症；原子力显微镜；杨氏模量

中图分类号：  文献标识码：A   doi:10.3969/j.issn.1000-6281.2021.01.007

 

A preliminary study on microscale stiffness of endometrial cells

LI Xia1, FENG Jian-tao2, REN Ke-li3, SUN Quan-mei3, ZHAO Xiao-ling4, HAN Dong3, KONG Wei-min4*

(1. Beijing Aerospace General Hospital, Beijing 100076; 2. Artemisinin Research Center, China Academy of Chinese Medical Sciences, Beijing 100700; 3. National Center for Nanoscience and Technology, BeiJing 100190; 4. Department of Gynecologic Oncology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100006, China)

Abstract Objectives: The mechanical model of endometrial cell was established based on atomic force microscopy (AFM), and the microscale stiffness of endometriosis was compared with endometrial cells in order to further study the pathogenesis of endometriosis. Methods: Endometrial tissue and the wall of a chocolate cyst of Ems were acquired,the primary culture of endometriosis tissue was carried out, AFM was used to obtain the cell force curve, and the apparent young's modulus of the cell calculated. Results: Young's modulus of locational glandular epithelial cells is higher than that of the ectopic glandular epithelial cells (2.96±1.48 kPa, 1.47±1.15 kPa, P<0.05); Young's modulus of stromal cells of normal position is compared with young's modulus of stromal cells of abnormal position, and there are no obvious change (3.93±1.50kPa, 3.14±1.65kPa, P>0.05). Conclusions: These findings suggest that the mechanical properties are different between the two kinds of cells, the stiffness of ectopic epithelial cells is much smaller, and the small cell stiffness is more conducive to cell migration. Therefore, this study also supports the cell migration theory.

Keywords endometriosis; atomic force microscopy; Young’s modulus

 

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