基于聚焦离子束-扫描电子显微技术的雪松木质部具缘纹孔三维重构

基于聚焦离子束-扫描电子显微技术的雪松木质部具缘纹孔三维重构

王  静，王  杰， 郭  娟，冯  韵，李喜霞，张建国，

姜笑梅，殷亚方，李  姗^*

(1.陕西科技大学, 环境科学与工程学院，陕西 西安 710021；2.中国林业科学研究院木材工业研究所，北京 100091；3.中国科学院生物物理所生物成像中心，北京 100101)

摘  要  木本植物木质部具缘纹孔是木质部水分输导、栓塞形成、扩散及疲劳产生的关键部位，同时也是影响木材干燥与防腐等加工工艺的重要构造特征。目前，针对具缘纹孔结构的成像技术大多集中在二维尺度，难以精确地反映其三维结构的相关信息。本文利用聚焦离子束-扫描电子显微镜，通过对雪松木质部管胞具缘纹孔进行连续切片和图像采集，并采用 Imaris软件重构其三维结构，分辨率达 5 nm。聚焦离子束-扫描电子显微技术实现了具缘纹孔三维结构的精准成像，能够反映具缘纹孔膜三维孔隙结构，有助于从微纳尺度揭示木质部水分输导、栓塞形成、扩散及疲劳的机制，并为木材干燥和保护提供科学依据。

关键词 雪松；具缘纹孔；木质部；三维重构

中图分类号：Q944.5；S718.47；S781.1； X173  

文献标识码：Adoi:10.3969/j.issn.1000-6281.2022.01.011

The three-dimensional structure of bordered pit in xylem of Cedrus deodara base on focused ion beam scanning electron microscopy

WANG Jing¹，WANG Jie²，GUO Juan²，FENG Yun³，LI Xi-xia³，ZHANG Jian-guo³，JIANG Xiao-mei²，

YIN Ya-fang²，LI Shan^1，2*

（1. Shaanxi University of Science & Technology, Xi’an 710021, China；2.Research Institute of Wood Industry，Chinese Academy of Forestry，Beijing 100091；3.Bioimaging Center, Institute of Biophysics, Chinese Academy of Sciences，Beijing 100101）

Abstract    Bordered pits in plant xylem are the key locations where xylem embolism forms, spreads and xylem fatigue occurs, and are also important microstructures affecting thedrying and preserving processes of the wood. At present, the imaging techniques of bordered pit structures are mostly concentrated in the two-dimensional scale, which could hardly reflect accurately the relevant information of the three-dimensional structure of bordered pits. In this paper, we adopted the focused ion beam scanning electron microscopy (FIB-SEM) to cut serial sections of bordered pits in xylem of Cedrus deodara, moreover, we reconstructed the three-dimensional structures of bordered pits using Imaris software with a maximum resolution of 5 nm. The focused ion beam scanning electron microscopy technique achieves accurate imaging of the three-dimensional structure of bordered pit and can reflect the three-dimensional structure of bordered pits membrane, which helps to reveal the mechanism of xylem embolism formation, spread and fatigue from the micro-nano scale and provides theoretical reference materials for wood drying and protection.

Keywords    Cedrus deodara; bordered pits; xylem embolism; three-dimensional reconstruction

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