家兔骨骼肌样品染色技术的改进
毛颖秋，张 欣，袁悦莹，卢建秋家兔骨骼肌样品染色技术的改进
毛颖秋，张 欣，袁悦莹，卢建秋*
（北京中医药大学科研实验中心，北京 100029）
摘 要：采用常规染色方法所制备的家兔骨骼肌样品，其图像中易出现小黒斑。本文在常规染色方法基础上进行改良，有效减少了黑斑的出现，其超微结构清晰，反差也有所增强。
关键词：透射电镜；家兔骨骼肌；NaOH
中图分类号：Q336 文献标识码：A doi:10.3969/j.1000-6281.2013.06.009
Improvement of staining method for rabbit skeletal muscle sample using transmission electron microscopy
MAO Ying-qiu，ZHANG Xin，YUAN Yue-ying
(Center of Science Experiment in Beijing University of Chinese Medicine, Beijing100029，China)
Abstract：Some black spots are common seen in the images of rabbit skeletal muscle tissue by using traditional staining method. In this paper, the staining method was improved, resulting in reducing black spots, much clearer ultra microstructure, and enhancing contrast grade.
Keywords: transmission electron microscope；skeletal muscle；NaOH
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