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冷冻扫描电镜制样方法及其在含水样品中的应用

莫家媚，张少鸿*，卢 思，付 娟，陈晓丽，苏秋成
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2024年 第43卷 第2期: 1000-6281（2024）02-231-09     下载地址：

                             冷冻扫描电镜制样方法及其在含水样品中的应用

莫家媚，张少鸿*，卢  思，付  娟，陈晓丽，苏秋成

（中国科学院广州能源研究所广东省新能源和可再生能源研究开发与应用重点实验室，广东广州  510640）

摘  要  常规扫描电镜要求所观察的样品必须干燥、无水，而一些含水样品在干燥过程中会发生结构变化，无法真实反映样品的结构信息。冷冻扫描电镜无需对样品进行干燥处理，可以直接分析含水样品表面、界面和内部结构，是研究高度含水样品的强有力工具。样品的制样方法在冷冻扫描电镜技术中占有重要地位，它直接关系到样品真实形貌特征的反映和对超微结构的正确解读。本文综述了冷冻扫描电镜的制样方法，并阐述冷冻扫描电镜技术在天然气水合物、微生物、乳液、水凝胶、动植物等含水样品中的应用。

关键词 冷冻扫描电镜；制样方法；含水样品；应用

中图分类号：R944.5；R94；R98；Q336  文献标识码：A         doi:10.3969/j.issn.1000-6281.2024.02.014

   

Sample preparation by cryo-scanning electron microscopy and its application in hydrated samples

 MO Jiamei，ZHANG Shaohong*，LU Si，FU Juan，CHEN Xiaoli，SU Qiucheng

(Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou Guangdong 510640, China)

Abstract  The sample studied by conventional scanning electron microscope should be in dried state. However, the drying process of a sample can result in the change of its microstructure. Cryo-scanning electron microscopy (Cryo-SEM) is a powerful tool for studying highly hydrated samples because it can directly analyze the surface, interface and internal structure of water-contained samples without drying the samples. The sample preparation method plays an important role in Cryo-SEM, which directly affect the observation effect and the interpretation of an image. In this work, the sample preparation method of Cryo-SEM and the application of gas hydrate, microbial, emulsions, hydrogels, plant, animal were described.

Keywords  Cryo-scanning electron microscopy (Cryo-SEM); sample preparation method; hydrated sample; application

 

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