阶梯式包埋板在肾活检电镜标本制备中的应用
侯晓涛,王 伶,钟学军,朱伟城,陈观庆,陈启晴,欧洁云,李福华,谢 琳,李依晨,叶慧琳,毕振海,杨惠霏*,官 阳*,沈婉君*阶梯式包埋板在肾活检电镜标本制备中的应用
侯晓涛,王 伶,钟学军,朱伟城,陈观庆,陈启晴,欧洁云,李福华,谢 琳,李依晨,叶慧琳,毕振海,杨惠霏*,官 阳*,沈婉君*
(1.广州金域医学检验中心,广东 广州 510000;2.暨南大学附属复大肿瘤医院,广东 广州510000;3.武汉大学人民医院超微病理中心,湖北 武汉430060;4.解放军总医院第一医学中心肾脏病医学部,北京100853)
摘 要 本文介绍了一款新型的阶梯式电镜标本包埋板,此包埋板具有独特的阶梯式设计,如应用于肾活检电镜标本的制备,较传统方法能节省环氧树脂和修块刀片的消耗量,且节约了修块、定位和半薄切片所需的时间。此包埋板成本低,操作简便,是高效的电镜标本包埋工具,为肾活检病理诊断提供有力支撑。此包埋板同样适用于其它组织、器官临床穿刺类标本和科研生物电镜标本的包埋。
关键词 阶梯式;包埋板;包埋板肾活检;电镜标本
中图分类号:Q34;R33;R361 文献标识码:B doi:10.3969/j.issn.1000-6281.2023.03.012
Application of stepped embedding plate in the preparation of electron microscope specimens for renal biopsy
HOU Xiao-Tao1,WANG Ling1,ZHONG Xue-Jun1,ZHU Wei-cheng1,CHEN Guan-qing1,CHEN Qi-qing1,OU Jie-yun1,LI Yi-chen1,YE Hui-lin1,BI Zhen-hai1,YANG Hui-Fei2,GUAN Yang3*,SHEN Wan-jun 4*
(1.Guangzhou kingmed diagnostics Center , Guangdong Guangzhou 510000;2.Fuda Cancer Hospital, Jinan University, Guangdong Guangzhou 510515; 3.Ultrastructural Pathology Center, Renmin Hospital of Wuhan University, Wuhan Hubei 430060;4.Department of Nephrology, First Medical Center of Chinese PLA General Hospital,Beijing 100853)
Abstract This paper introduces a new type of stepped electron microscope specimen embedding plate. The embedding plate has a unique stepped design. If it is used in the preparation of kidney biopsy electron microscope specimens, it can save the consumption of epoxy resin and trimmingblades comparing with traditional methods. It also saves time required for trimming, positioning and semi-thin sectioning. The embedding plate has the advantages of low cost and simple operation, and it is an efficient tool for embedding electron microscope specimens, which provides a strong support for pathological diagnosis of renal biopsy. The embedding plate is also suitable for embedding clinical puncture specimens of other tissues and organs as well as scientific research bio-electron microscope specimens.
Keywords stepped; embedded plate; renal biopsy; electron microscope specimen
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